Microinjection of traceable LPS into the brain leads to drainage of LPS molecules into circulation, and causes active infiltration of peripheral macrophages into the liver. (a) Schematic. (b) Whole-body in vivo imaging analysis. Dotted line demarcates the liver.
In vivo time-lapse imaging tracking macrophages (green) infiltrating the liver (red) in real time in the 4 dpf zebrafish larvae. Left, merged channels; Right, macrophages only (green channel).
Coordination and intimate contacts between macrophages and neutrophils are prevalent around the liver in the normal living zebrafish. Video shows time-lapse confocal imaging of macrophages (green) and neutrophils (red).
The Celia Shiau Lab 